PHASTEST (PHAge Search Tool with Enhanced Sequence Translation) is a web server designed to support the rapid identification, annotation and visualization of prophage sequences within bacterial genomes and plasmids. PHASTEST also supports extensive annotation and interactive visualization of all other genes (protein coding regions, tRNA sequences and rRNA sequences) in those same genomes. PHASTEST can now process a typical bacterial genome in 3.2 minutes from the raw sequence alone or in 1.3 minutes when given a pre-annotated GenBank file. PHASTEST is 30.5% faster than PHASTER. PHASTEST not only offers faster and more accurate prophage annotations than its predecessors (PHAST and PHASTER), but it also provides more complete whole genome annotations and much improved genome visualization capabilities. PHASTEST now offers a much more responsive, interactive visualization interface that allows users to generate, edit, annotate and interactively visualize (zooming, rotating, dragging, panning, resetting) publication quality genome maps (both circular and linear versions).

Please cite PHASTEST:
Wishart, D.S., Han, S., Saha, S., Oler, E., Peters, H., Grant, J., Stothard, P., Gautam, V. (2023), PHASTEST: Faster than PHASTER, Better than PHAST, Nucleic Acids Research (Web Server Issue),

Please also cite:
PHASTER: Arndt, D., Grant, J., Marcu, A., Sajed, T., Pon, A., Liang, Y., Wishart, D.S. (2016) PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res., 44(Web Server issue): W16-W21
PHAST: Zhou, Y., Liang, Y., Lynch, K.H., Dennis, J.J. and Wishart, D.S. (2011) PHAST: a fast phage search tool. Nucleic Acids Res., 39, W347-W352.

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